1. Placement

Hold the mirror arm with your right hand and the mirror base with your left hand, keeping the mirror body upright. The desktop should be clean and stable, and a window facing or well lit area should be chosen. The single tube is usually placed on the left side, 3-4 centimeters away from the table edge.

2. Cleaning

3. Aiming light

Raise the lens tube to a distance of 1-2 centimeters from the stage, and align the low-power lens with the light hole. Adjust the aperture and reflector. When the light is strong, use a flat mirror. When the light is weak, use a concave mirror. The reflector should be rotated with both hands.

4. Install specimen

5. Focusing

Operation note: Do not focus directly under a high-power lens; When the lens tube descends, the distance between the lens tube and the specimen should be observed from the side; To understand the critical value of object distance.

6. Observation

Adjustment of light intensity: In general, the light should be strong for stained specimens, and weak for colorless or unstained specimens; The light should be weak when observing with a low-power lens, and strong when observing with a high-power lens. In addition to adjusting the reflector or light source, the adjustment of the iris aperture is also very important.

(1) Low magnification observation

When observing any specimen, it is necessary to first use a low-power microscope because its field of view is large, making it easy to detect the target and determine the area to be observed.

(2) High magnification observation

Do not use a coarse focusing knob when using a high-power lens, as it can easily crush the cover glass and damage the lens.

(3) Observation of oil mirror

First, use a low-power lens and a high-power lens to move the object to the center of the field of view, and then change the oil lens for observation. Before observing with an oil mirror, the brightness of the microscope should be adjusted to * bright and the aperture should be fully opened.

7. End operation

After observation, remove the sample, twist the converter, and make the V-shaped lens slant to both sides. The reflector should be upright, lower the lens tube, wipe it clean, and put on the lens cover.